IACUC / LARC STANDARD PROCEDURES

MHC-matched intravenous adoptive cell transfers

Description of procedure:
Adoptive Transfer Protocol:  A donor mouse is euthanized by UCSF standard procedures, and lymphoid cells from donor mice are prepared as a single cell suspension and adjusted to an appropriate final volume with sterile PBS.  Each mouse will receive 0. 1- 0.4 ml of cell suspension.  Cell numbers may vary between 1 x 10^4 and \1 x 10^8 cells.  Recipient mice are injected i.v. via tail vein or anesthetized and injected retro-orbitally using a small gauge (i.e. 30g) needle.  If anesthetized, mice will be monitored continuously until ambulatory.

Mice will be evaluated for general appearance, ability to move normally around cage and reach food and water, ability to eat and drink, grooming habits, skin color, lethargy, aggression (animals will be housed individually if aggressive behavior is shown).

Literature search words required:

Literature search was performed for refinement of this Standard Procedure on Sept 1, 2006 :

Key Words	Search Site	Years Covered
Adoptive lymphocyte transfer	Pubmed	1963-2009
Adoptive lymphocyte transfer	Google	No limit

Agents:

• This procedure requires anesthetic for retro-orbital injection. All agents administered to animals should be listed in the “Agents” section of the protocol in RIO.

Adverse Effects:

Adverse Effects
Procedure, Agent or Phenotype	Potential Adverse Effects	Management
retrooribital injections	trauma to the eye	euthanasia
Cell transfer	Graft vs. host disease	euthanasia

Monitoring Parameters:

Monitoring Parameters
Monitoring Parameters	Frequency	PI/Lab will Document
General appearance/activity	2-3 times for 5 days then weekly thereafter	No
Ability to eat & drink	2-3 times for 5 days then weekly thereafter	No
Body condition score less than or equal to 2	2-3 times for 5 days then weekly thereafter	Yes

Describe the conditions, complications, and criteria (e.g. uncontrolled infection, loss of more than 15% body weight, etc.) that would lead to removal of an animal from the study, and describe how this will be accomplished (e.g. stopping treatment, euthanasia).

Mice with Body Condition score less than or equal to 2 will be euthanized.

For all investigators housing animals with tumor formation, skin lesions, neurological deficits, or that are in Category E, list the expected characteristics/clinical presentations and endpoints of the animal model and the criteria for euthanasia. Note: The IACUC also requires such lists to be posted in the respective animal rooms and monitored by the IACUC compliance staff and LARC, to assure PI adherence to the endpoints listed.