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IACUC / LARC STANDARD PROCEDURES
Glucose Monitoring of Blood in Mice (Posted May 2008)
Description of procedure:
Blood glucose levels will be monitored on blood obtained from mice by tail snipping.
- Mice that are older than 3-weeks of age will be anesthetized with isoflurane for the initial tail snip.
- Snip tail using a clean razor blade or sharp scissors.
- Gently milk blood from tail. Place one droplet of blood (<25 µls) on a glucose test strip and read using a glucometer.
- For successive weekly sampling, remove the previous scab, milk the tail and collect blood as before. Re-snipping is unnecessary for successive sampling. Anesthetics are not required for subsequent blood sampling because only the scab of the initial snip is removed.
- Apply direct pressure to achieve hemostasis. Use silver nitrate as needed.
- Monitor mice continuously after isoflurane anesthesia until mouse is ambulatory.
- Evaluate mice for general appearance and hemostasis before returning to the home cage.
Literature search words required:
Literature search was performed for refinement of this Standard Procedure on August 15, 2007:
Key Words |
Search Site |
Years Covered |
Glucose monitoring, diabetes, mice, blood collection in rodents |
Pubmed |
1958-May 2008 |
Agents:
This procedure requires isoflurane for mice older than 3 weeks of age for initial snip. All agents administered to animals should be listed in the “Agents” section of RIO
Adverse Effects:
Adverse Effects |
Procedure, Agent or Phenotype |
Potential Adverse Effects |
Management |
Tail tip snip |
uncontrolled bleeding, infection |
Direct pressure/silver nitrate, consult with a LARC veterinarian |
Monitoring Parameters |
Monitoring Parameters |
Frequency |
PI/Lab will Document |
General appearance/activity level/assure blood flow has stopped. |
Once within the first hour after each collection procedure |
No |
Describe the conditions, complications, and criteria (e.g. uncontrolled infection, loss of more than 15% body weight, etc.) that would lead to removal of an animal from the study, and describe how this will be accomplished (e.g. stopping treatment, euthanasia). |
If mice develop diabetes, they will be implanted with an insulin pellet- see the SOP for implantation of insulin pellets for this section. |
For all investigators housing animals with tumor formation, skin lesions, neurological deficits, or that are in Category E, list the expected characteristics/clinical presentations and endpoints of the animal model and the criteria for euthanasia. Note: The IACUC also requires such lists to be posted in the respective animal rooms and monitored by the IACUC compliance staff and LARC, to assure PI adherence to the endpoints listed. |
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