IACUC / LARC STANDARD PROCEDURES

THIOGLYCOLLATE INDUCTION OF ACTIVATED MACROPHAGES AND GRANULOCYTES IN MICE (Posted May 2007)

Description of procedure:

Mice will be injected intraperitoneally with ≤ 3ml of sterile thioglycollate medium (3% w/v of an autoclaved stock prepared from dehydrated thioglycollate medium and water*; 2-3 ml for mice that are ≥ 2 months old; 1-1.5ml for mice that are < 2 months old). The mice will be euthanized for recovery of peritoneal inflammatory cells at various time points following injection. Leukocytes will accumulate rapidly in normal mice in response to the injection (e.g., large numbers of cells can be recovered after 24-48 hours). Some strains may require up to 4 days to produce adequate numbers of cells, so the study will last 1-4 days post-injection, after which animals are euthanized. The procedure can be used as a means to recover activated inflammatory leukocytes from mice, or it can be used as an analytical procedure to compare rates of leukocyte accumulation in different strains of mice.

The treated mice will be monitored on a daily basis for signs of discomfort. Signs of discomfort would include hunched posture, ruffled fur and lack of movement around the cage. The body condition score index will also be used to evaluate the welfare of the mice. If the mice show signs of discomfort, or a BCS < 2, they should be euthanized.

Literature search words required:

Literature search was performed for refinement of this Standard Procedure on March 9, 2007

Key Words	Search Site	Years Covered
Thioglycollate induction in mice	Pubmed	1977-2006

Agents:

All agents administered to animals should be listed in the "Agents" section of RIO.

Adverse Effects:

Procedure, Agent or Phenotype	Potential Adverse Effects	Management
Ip injection of thioglycollate	Weight loss, lethargy, Peritonitis	Euthanasia

Monitoring Parameters:

Monitoring Parameters	Frequency	PI/Lab will Document
General appearance/activity	Daily for up to 4 days	No
Body condition scoring < 2	Daily for up to 4 days	Yes

Describe the conditions, complications, and criteria (e.g. uncontrolled infection, loss of more than 15% body weight, etc.) that would lead to removal of an animal from the study, and describe how this will be accomplished (e.g. stopping treatment, euthanasia).

Animals with a BCS of less than 2 will be euthanized.

For all investigators housing animals with tumor formation, skin lesions, neurological deficits, or that are in Category E, list the expected characteristics/clinical presentations and endpoints of the animal model and the criteria for euthanasia. Note: The IACUC also requires such lists to be posted in the respective animal rooms and monitored by the IACUC compliance staff and LARC, to assure PI adherence to the endpoints listed.

N/A.