• Too much blood collected at any one time may cause hypovolemic shock, physiological stress and even death. If smaller volumes are collected too frequently, anemia may result.
• As a general rule, 10% of the total blood volume can be collected at one time every 2-4 weeks. Or 1% at intervals of 24 hours or more. Total blood volume can be calculated as approximately 7.5% of body weight.
• As a general rule, 10% of the total blood volume can be collected at one time every 2-4 weeks. Or 1% at intervals of 24 hours or more. Total blood volume can be calculated as approximately 7.5% of body weight.

EXAMPLE

Average Adult Weight	Total Blood Volume	Maximum Sample in mls q 2-4 Weeks	Estimated Exsanguination Volume
50 kg	3750 ml	375 ml	1870 ml

• Use aseptic technique.
• If you are not experienced in blood collection techniques, training is available.

Collection Site	Advantages	Disadvantages
Jugular Vein	Maximum quantities of blood can be collected Anesthesia not required	Requires specialized training Requires two people
Cephalic Vein	Collection of small to moderate volumes of blood Anesthesia not required	Requires two people Training recommended
Lateral Saphenous Vein	Collection of small to moderate volumes of blood Anesthesia not required	Requires two people Training recommended